Not sure if it works on vista. I am using a dedicated XP box for it and it worked great. I haven't retried the vista machine since the original 18 meg installed glitch. I know that the drivers can be an issue....do a clean install of mdx and when you plug in the brain, go to device manager and make sure it is using the appropriate driver. Hope this helps.
Yes it works on Vista x86 and XP x86. No 64bit OS. My developement machine is Vista Ultimate x86.