YES! I'm running a 3.5" desktop hard drive. Sorry I don't have all the brand name/ rpm info but I can tell you it's name brand Seagate, Maxtor or someone, and it's the faster speed. 40gb.
It's been running for three months with no problems. I've driven it over unpaved washboard roads in Arizona and the crater-like pot hole infested roads of Flint, MI. No problems so far. Nothing special for suspension, just a lexan case... but maybe that is special.